Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | RPA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, Replication protein A 70 kDa DNA-binding subunit, N-terminally processed, RPA1, REPA1, RPA70 |
| Product description | RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.1) Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13660-13665(2010) |
| Immunogen | This RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the C-terminal region of human RPA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPA1 |
|---|---|
| Alternative Names | Replication protein A 70 kDa DNA-binding subunit RP-A p70 Replication factor A protein 1 RF-A protein 1 Single-stranded DNA-binding protein Replication protein A 70 kDa DNA-binding subunit N-terminally processed RPA1 REPA1 RPA70 |
| Molecular Weight(MW) | 68 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
