Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Neuro-2a whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Neuro-2a whole cell lysate Lane 4: human placenta lysate Lysates/proteins at 20 ug per lane.| Product Name | RPL22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 60S ribosomal protein L22, EBER-associated protein, EAP, Epstein-Barr virus small RNA-associated protein, Heparin-binding protein HBp15, RPL22 |
| Product description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.1) Houmani, J.L., et al. J. Virol. 83(19):9844-9853(2009) |
| Immunogen | This RPL22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the C-terminal region of human RPL22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPL22 |
|---|---|
| Alternative Names | 60S ribosomal protein L22 EBER-associated protein EAP Epstein-Barr virus small RNA-associated protein Heparin-binding protein HBp15 RPL22 |
| Molecular Weight(MW) | 15 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Neuro-2a whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Neuro-2a whole cell lysate Lane 4: human placenta lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
