Application
RPL23 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Product Name | RPL23 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 60S ribosomal protein L23, 60S ribosomal protein L17, RPL23 |
| Product description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.1) Gehring, N.H., et al. Cell 137(3):536-548(2009) Wanzel, M., et al. Nat. Cell Biol. 10(9):1051-1061(2008) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) Jin, A., et al. Mol. Cell. Biol. 24(17):7669-7680(2004) |
| Immunogen | This RPL23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the Central region of human RPL23. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPL23 |
|---|---|
| Alternative Names | 60S ribosomal protein L23 60S ribosomal protein L17 RPL23 |
| Molecular Weight(MW) | 15 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
RPL23 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
