Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Hela,A2058 cell line lysates (15ug/lane).Application
RPS3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | RPS3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255|HAMAP-Rule:MF_03122}, FTE1, MFTL |
| Product description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.1) Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008) |
| Immunogen | This RPS3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the C-terminal region of human RPS3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS3A |
|---|---|
| Alternative Names | 40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122} v-fos transformation effector protein Fte-1 RPS3A {ECO:0000255|HAMAP-Rule:MF_03122} FTE1 MFTL |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Hela,A2058 cell line lysates (15ug/lane).
Application
RPS3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
