Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: rat lung lysate Lane 6: mouse lung lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | RPS4X Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 40S ribosomal protein S4, X isoform, SCR10, Single copy abundant mRNA protein, RPS4X, CCG2, RPS4, SCAR |
| Product description | 1) Fisher E.M.C.,et al.Cell 63:1205-1218(1990). |
| Immunogen | This RPS4X antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 209-243 amino acids from the human region of human RPS4X. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS4X |
|---|---|
| Alternative Names | 40S ribosomal protein S4 X isoform SCR10 Single copy abundant mRNA protein RPS4X CCG2 RPS4 SCAR |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: rat lung lysate Lane 6: mouse lung lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
