Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of RRAGD Antibody in MDA-MB435 cell line lysates (35ug/lane)Application
Western blot analysis of RRAGD Antibody in mouse cerebellum tissue lysates (35ug/lane)Application
RRAGD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RRAGD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ras-related GTP-binding protein D, Rag D, RagD, RRAGD ( HGNC:19903 ) |
| Product description | RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.1) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
| Immunogen | This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RRAGD |
|---|---|
| Alternative Names | Ras-related GTP-binding protein D Rag D RagD RRAGD ( HGNC:19903 ) |
| Molecular Weight(MW) | 46 kDa |
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of RRAGD Antibody in MDA-MB435 cell line lysates (35ug/lane)
Application
Western blot analysis of RRAGD Antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
RRAGD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
