Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).Application
RSBN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: MCF-7 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | RSBN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Round spermatid basic protein 1, RSBN1 |
| Product description | 1) Rose, J. Phd, et al. Mol. Med. (2010) In press : |
| Immunogen | This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RSBN1 |
|---|---|
| Alternative Names | Round spermatid basic protein 1 RSBN1 |
| Molecular Weight(MW) | 90 kDa |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application
RSBN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: MCF-7 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
