Application
Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.Application
Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) and human liver carcinoma (bottom) showing nuclear location using RSK1 antibody with DAB staining.| Product Name | RSK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPKAPK1A, RSK1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1, RSK, HU-1, MAPKAPK1A |
| Product description | Rsk1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK proteins possess two separate kinase domains, the C terminal kinase domain and the N terminal kinase domain, separated by a linker region containing a hydrophobic motif. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation.1) Alcorta, D.A., et al. 1989. Mol. Cell. Biol. 9:3850-3859. |
| Immunogen | Ni-NTA purified truncated recombinant RSK1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | RPS6KA1 |
|---|---|
| Alternative Names | MAPKAPK1A RSK1 90 kDa ribosomal protein S6 kinase 1 S6K-alpha-1 RSK HU-1 MAPKAPK1A |
Application
Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.
Application
Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) and human liver carcinoma (bottom) showing nuclear location using RSK1 antibody with DAB staining.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | RSK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
