Application
Western blot analysis of extracts from HepG2 cells (Lane 2), using RSK1/2/3/4 (Phospho-Ser221/227/218/232) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | RSK1/2/3/4 (phospho Ser221/227/S218/232) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KS6A1, KS6AA, MAPKAP-K1a, RSK1 |
| Product description | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway.1) Moller D.E., Am. J. Physiol. 266:C351-C359(1994). |
| Immunogen | RSK1/2/3/4 (phospho Ser221/227/S218/232) antibody was raised against a peptide sequence around phosphorylation site of Serine 221/227/218/232 (A-Y-S (p)-F-C) derived from Human RSK1/2/3/4. |
| Modification | p-Ser221/227/S218/232 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KA1, RPS6KA2, RPS6KA3, RPS6KA6 |
|---|---|
| Alternative Names | KS6A1 KS6AA MAPKAP-K1a RSK1 |
| Molecular Weight(MW) | 85 kDa |
| Tissue Specificity | The antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232. |
| Entrez Gene | 6195/6197/6196/27330 |
|---|---|
| Protein Accession | NP_002944.2 |
Application
Western blot analysis of extracts from HepG2 cells (Lane 2), using RSK1/2/3/4 (Phospho-Ser221/227/218/232) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚,C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
