Application
Immunohistochemistry of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 μg/ml.Application
Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Application
Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Application
Immunofluorescence of RSPO1 in Human Spleen tissue with RSPO1 antibody at 20 μg/mL.Application
Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 μg/mL.Application
Western blot analysis of RSPO1 in human heart tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | RSPO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RSPO1 Antibody: RSPO, CRISTIN3, R-spondin-1, Roof plate-specific spondin-1, hRspo1 |
| Product description | RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.1) Kamata T, Katsube K, Michikawa M, et al. R-spondin, a novel gene with thrombospondin type I domain, was expressed in the dorsal neural tube and affected Wnts mutants. Biochim. Biophys. Acta 2004; 1676:51-61. |
| Immunogen | RSPO1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RSPO1 antibody can be used for detection of RSPO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RSPO1 |
|---|---|
| Alternative Names | RSPO1 Antibody: RSPO CRISTIN3 R-spondin-1 Roof plate-specific spondin-1 hRspo1 |
| Molecular Weight(MW) | Predicted: 20, 29 kDa Observed: 26 kDa |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members. |
| Entrez Gene | 284654 |
|---|---|
| Protein Accession | NP_001033722 |
Application
Immunohistochemistry of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 μg/ml.
Application
Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.
Application
Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.
Application
Immunofluorescence of RSPO1 in Human Spleen tissue with RSPO1 antibody at 20 μg/mL.
Application
Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 μg/mL.
Application
Western blot analysis of RSPO1 in human heart tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | RSPO1 antibody can be used for detection of RSPO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RSPO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide. |
