Application
Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 μg/mL.Application
Immunofluorescence of RSPO3 in 293 cells with RSPO3 antibody at 20 μg/mL.Application
Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.| Product Name | RSPO3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | R-spondin-3, CRISTIN1, PWTSR, THSD2 |
| Product description | RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis (1,2). RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development (3,4).1) Chen JZ, Wang S, Tang R, et al. Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR. Mol. Biol. Rep. 2002; 29:287-92. |
| Immunogen | RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3. The immunogen is located within amino acids 200 - 250 of RSPO3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RSPO3 |
|---|---|
| Alternative Names | R-spondin-3 CRISTIN1 PWTSR THSD2 |
| Molecular Weight(MW) | Predicted: 30 kDa Observed: 30 kDa |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | RSPO3 antibody is human and mouse reactive. At least three isoforms of RSPO3 are known to exist; this antibody will detect all three. |
Application
Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 μg/mL.
Application
Immunofluorescence of RSPO3 in 293 cells with RSPO3 antibody at 20 μg/mL.
Application
Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RSPO3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RSPO3 antibody is supplied in PBS containing 0.02% sodium azide. |
