Application
Western blot analysis of RTN4RL2 in rat brain tissue lysate with RTN4RL2 antibody at 1 μg/mL.| Product Name | RTN4RL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RTN4RL2 Antibody: NgR2, NGRH1, NGRL3, Reticulon-4 receptor-like 2, Nogo receptor-like 3, NgR2 |
| Product description | RTN4RL2 Antibody: Reticulon 4 receptor-like 2 (RTN4RL2), also known as NgR2and NgRH1, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- and C-termini. The membrane anchored RTN4RL2 can be solubilized through the action of phospholipase or an unidentified MTMMP to generate a 46 kDa soluble receptor. It has been suggested that RTN4RL2 may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.1) Pignot V, Hein AE, Barske C, et al. Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the Nogo-66 receptor. J. Neurochem. 2003; 85:717-28. |
| Immunogen | Rabbit polyclonal RTN4RL2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RTN4RL2. The immunogen is located within amino acids 330 - 380 of RTN4RL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RTN4RL2 antibody can be used for detection of RTN4RL2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RTN4RL2 |
|---|---|
| Alternative Names | RTN4RL2 Antibody: NgR2 NGRH1 NGRL3 Reticulon-4 receptor-like 2 Nogo receptor-like 3 NgR2 |
| Molecular Weight(MW) | Predicted: 46 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%) |
| Tissue Specificity | Three alternatively spliced transcript variants have been observed. RTN4RL2 antibody is predicted to not cross-react with other Nogo receptor-like proteins. |
Application
Western blot analysis of RTN4RL2 in rat brain tissue lysate with RTN4RL2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | RTN4RL2 antibody can be used for detection of RTN4RL2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RTN4RL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RTN4RL2 Antibody is supplied in PBS containing 0.02% sodium azide. |
