Application
Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.Application
Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.Application
Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.| Product Name | Rubicon Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226 |
| Product description | Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).1) Matsunaga K, Saitoh T, Tabata K, et al. Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Nat. Cell Biol. 2009; 11:385-96. |
| Immunogen | Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIAA0226 |
|---|---|
| Alternative Names | Run domain Beclin-1 interacting and cysteine-rich containing protein KIAA0226 |
| Molecular Weight(MW) | Predicted: 90, 101, 109 kDa Observed: 91, 99 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist. |
| Entrez Gene | 9711 |
|---|---|
| Protein Accession | XP_005269431 |
Application
Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.
Application
Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.
Application
Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Rubicon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Rubicon antibody is supplied in PBS containing 0.02% sodium azide. |
