Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Product Name | RUNX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX2, CCD, AML3, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1 |
| Product description | RUNX2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis, acting as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants, encoding different protein isoforms, result from alternate promoter use as well as alternate splicing.1) Guo, J., et al., (2006) Dev. Biol. 292 (1), 116-128. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUNX2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:1000 ELISA:1:5000~20000 |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RUNX2 |
|---|---|
| Alternative Names | RUNX2 CCD AML3 CCD1 CLCD OSF2 CBFA1 OSF-2 PEA2aA PEBP2aA CBF-alpha-1 |
| Molecular Weight(MW) | 57 kDa, 55 kDa |
| Entrez Gene | 860 |
|---|---|
| Protein Accession | NP_001019801 |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:1000 ELISA:1:5000~20000 |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RUNX2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
