Application
Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL.| Product Name | RUSC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUSC1 Antibody: NESCA, NESCA, RUN and SH3 domain-containing protein 1, New molecule containing SH3 at the carboxy-terminus, Nesca |
| Product description | RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.1) Matsuda S, Miyazaki K, Ichigotani Y, et al. Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3. Biochim. Biophys. Acta2000; 1491:321-6. |
| Immunogen | RUSC1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RUSC1. The immunogen is located within amino acids 780 - 830 of RUSC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUSC1 |
|---|---|
| Alternative Names | RUSC1 Antibody: NESCA NESCA RUN and SH3 domain-containing protein 1 New molecule containing SH3 at the carboxy-terminus Nesca |
| Entrez Gene | 23623 |
|---|---|
| Protein Accession | NP_001098673 |
Application
Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RUSC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RUSC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
