Application
Immunohistochemistry of RUSC2 in human brain tissue with RUSC2 antibody at 5 μg/mL.Application
Immunofluorescence of RUSC2 in human brain tissue with RUSC2 antibody at 20 μg/mL.Application
Western blot analysis of RUSC2 in SK-N-SH cell lysate with RUSC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | RUSC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUSC2 Antibody: Iporin, KIAA0375, Iporin, Interacting protein of Rab1 |
| Product description | RUSC2 Antibody: RUSC2, also known as Iporin, shares with the related protein RUSC1 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC2 is a rab1-interacting protein that also interacts with GM130, another rab1-interacting protein. RUSC2 interacts with specific rab1 isoforms with different rab-binding specificity. It has been suggested that RUSC2 may function as a link between the targeting of ER derived vesicles triggered by the rab1 GTPase and a signaling pathway composed of proteins containing SH3 and/or poly-proline regions.1) Bayer M, Fischer J, Kremerskthen J, et al. Identification and characterization of Iporin as a novel interaction partner for rab1. BMC Cell Biol. 2005; 29:6:15. |
| Immunogen | RUSC2 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human RUSC2. The immunogen is located within amino acids 70 - 120 of RUSC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RUSC2 antibody can be used for detection of RUSC2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUSC2 |
|---|---|
| Alternative Names | RUSC2 Antibody: Iporin KIAA0375 Iporin Interacting protein of Rab1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms are known to exist; this antibody will detect all three isoforms. This antibody is predicted to not cross-react with RUSC1. |
Application
Immunohistochemistry of RUSC2 in human brain tissue with RUSC2 antibody at 5 μg/mL.
Application
Immunofluorescence of RUSC2 in human brain tissue with RUSC2 antibody at 20 μg/mL.
Application
Western blot analysis of RUSC2 in SK-N-SH cell lysate with RUSC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | RUSC2 antibody can be used for detection of RUSC2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RUSC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RUSC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
