Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on K562 at 1:200.| Product Name | RUVBL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUVBL1, ECP54, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49A, INO80H, PONTIN |
| Product description | RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.1) Jha, S., (2008) Mol. Cell. Biol. 28 (8), 2690-2700. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUVBL1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RUVBL1 antibody can be used for detection of RUVBL1 by ELISA at 1:312500. RUVBL1 antibody can be used for detection of RUVBL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUVBL1 |
|---|---|
| Alternative Names | RUVBL1 ECP54 NMP238 Pontin52 RVB1 TIH1 TIP49 TIP49A INO80H PONTIN |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on K562 at 1:200.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | RUVBL1 antibody can be used for detection of RUVBL1 by ELISA at 1:312500. RUVBL1 antibody can be used for detection of RUVBL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RUVBL1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
