Application
Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | RWDD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RWDD3 Antibody: RSUME, RSUME, RWD domain-containing protein 3, RWD-containing sumoylation enhancer |
| Product description | RWDD3 Antibody: RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases IκBeta sumoylation and stability. In addition, RWDD3 inhibits TNF-α-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of IκBeta increased stability. RSUME-enhanced sumoylation of IκBeta leads to the inhibition of NF-κBeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.1) Carbia-Nagashima A, Gerez J, Perez-Castro C, et al. RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia. Cell2007; 131:309-23. |
| Immunogen | RWDD3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3. The immunogen is located within the last 50 amino acids of RWDD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RWDD3 |
|---|---|
| Alternative Names | RWDD3 Antibody: RSUME RSUME RWD domain-containing protein 3 RWD-containing sumoylation enhancer |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (93%) |
Application
Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RWDD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RWDD3 Antibody is supplied in PBS containing 0.02% sodium azide. |
