Application
Western blot analysis using S100 A antibody against truncated S100A recombinant protein.Application
Immunohistochemical analysis of paraffin - embedded human brain tissue showing cytoplasmic using S100A antibody with DAB staining.| Product Name | S100A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S100A, S-100 protein alpha chainS100, S100A, S100-alpha |
| Product description | S100 calcium binding protein A1 (S100-alpha/ S100A1), The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.1) Koenig A, Wojcieszyn J, Weeks BR, et al. Vet Pathol. 2001;38(4):427-35. |
| Immunogen | Ni-NTA purified truncated recombinant S100A expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| Dilution ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S100A1 |
|---|---|
| Alternative Names | S100A S-100 protein alpha chainS100 S100A S100-alpha |
Application
Western blot analysis using S100 A antibody against truncated S100A recombinant protein.
Application
Immunohistochemical analysis of paraffin - embedded human brain tissue showing cytoplasmic using S100A antibody with DAB staining.| Application Notes | Dilution ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | S100A monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
