Application
Immunohistochemistry of SAMHD1 (phospho Thr592) in human brain tissue with SAMHD1 (phospho Thr592) antibody at 2.5 μg/ml.Application
Immunofluorescence of phosphoSAMHD1 in human brain tissue with phosphoSAMHD1 antibody at 20 μg/mL.Application
Western blot analysis of SAMHD1 (phospho Thr592) in 293 cells transfected with (A) empty expression vector, (B) wild-type SAMHD1, (C) SAMHD1 (T592A) and (D) SAMHD1 (T592E) with SAMHD1 (phospho Thr592) antibody at 1 μg/ml.| Product Name | SAMHD1 (phospho Thr592) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88 |
| Product description | The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1). SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3). Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1).1) Rice GI, Bond J, Asipu A, et al. Mutations involved in Aicardi-Goutieres syndrome implicate SAMHD1 as regulator of the innate immune system. Nat. Genet. 2009; 41:829-32. |
| Immunogen | Phospho-SAMHD1 antibody was raised against a phosphorylated 18 amino acid peptide near the carboxy terminus of human Phospho-SAMHD1 surrounding T592. |
| Modification | p-Thr592 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| SAMHD1 (phospho Thr592) antibody can be used for detection of SAMHD1 (phospho Thr592) by western blot at at 0.5-1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SAMHD1 (phospho Thr592) |
|---|---|
| Alternative Names | SAM domain and HD domain 1 DCIP CHBL2 HDDC1 MOP-5 SBBI88 |
| Molecular Weight(MW) | Predicted: 69 kDa |
| Tissue Specificity | Phospho-SAMHD1 antibody is human and mouse reactive. Phospho-SAMHD1 antibody will only detect the phosphorylated form of SAMHD1. |
Application
Immunohistochemistry of SAMHD1 (phospho Thr592) in human brain tissue with SAMHD1 (phospho Thr592) antibody at 2.5 μg/ml.
Application
Immunofluorescence of phosphoSAMHD1 in human brain tissue with phosphoSAMHD1 antibody at 20 μg/mL.
Application
Western blot analysis of SAMHD1 (phospho Thr592) in 293 cells transfected with (A) empty expression vector, (B) wild-type SAMHD1, (C) SAMHD1 (T592A) and (D) SAMHD1 (T592E) with SAMHD1 (phospho Thr592) antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 10-301 - Human Brain Tissue Slide |
|---|---|
| Application Notes | SAMHD1 (phospho Thr592) antibody can be used for detection of SAMHD1 (phospho Thr592) by western blot at at 0.5-1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAMHD1 (phospho Thr592) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SAMHD1 (phospho Thr592) antibody is supplied in PBS containing 0.02% sodium azide. |
