Application
Antibody used in WB on Transfected 293T at 0.125 ug/ml.| Product Name | SAP18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAP18, SAP18P, 2HOR0202 |
| Product description | Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. SAP18 is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter.Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter.1) Zhang, Y., (1997) Cell 89 (3), 357-364. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SAP18. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| SAP18 antibody can be used for detection of SAP18 by ELISA at 1:312500. SAP18 antibody can be used for detection of SAP18 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Dog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SAP18 |
|---|---|
| Alternative Names | SAP18 SAP18P 2HOR0202 |
| Molecular Weight(MW) | 18 kDa |
Application
Antibody used in WB on Transfected 293T at 0.125 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | SAP18 antibody can be used for detection of SAP18 by ELISA at 1:312500. SAP18 antibody can be used for detection of SAP18 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SAP18 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
