Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Product Name | SAP30 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAP30, |
| Product description | Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. SAP30 is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.1) Sironi, E., (2004) J. Cutan. Pathol. 31 (4), 318-322. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SAP30. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SAP30 antibody can be used for detection of SAP30 by ELISA at 1:312500. SAP30 antibody can be used for detection of SAP30 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SAP30 |
|---|---|
| Alternative Names | SAP30 |
| Molecular Weight(MW) | 23 kDa |
Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | SAP30 antibody can be used for detection of SAP30 by ELISA at 1:312500. SAP30 antibody can be used for detection of SAP30 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SAP30 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
