Application
Immunofluorescence of SAPAP2 in Human Brain cells with SAPAP2 antibody at 20 μg/mL.| Product Name | SAPAP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAPAP2 Antibody: DAP2, SAPAP2, DAP2, Disks large-associated protein 2, DAP-2 |
| Product description | SAPAP2 Antibody: SAP90/PSD-95-associated protein 2 (SAPAP2, also known as DLGAP2) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP2 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP2 mRNA is targeted to cell bodies in a similar manner to SAPAP1 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies.1) SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem.1997; 272:11943-51. |
| Immunogen | SAPAP2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human SAPAP2. The immunogen is located within amino acids 480 - 530 of SAPAP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SAPAP2 antibody can be used for detection of SAPAP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLGAP2 |
|---|---|
| Alternative Names | SAPAP2 Antibody: DAP2 SAPAP2 DAP2 Disks large-associated protein 2 DAP-2 |
| Molecular Weight(MW) | Predicted: 43 kDa Observed: 52 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms of SAPAP2 are known to exist. This SAPAP2 antibody will not cross-react with other SAPAP proteins. |
Application
Immunofluorescence of SAPAP2 in Human Brain cells with SAPAP2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | SAPAP2 antibody can be used for detection of SAPAP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAPAP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SAPAP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
