Application
Western blot analysis of SAPAP3 in rat brain tissue lysate with SAPAP3 antibody at (A) 1 (B) 2 μg/mL.Application
Immunofluorescence of SAPAP3 in human brain tissue with SAPAP3 antibody at 20 μg/mL.Application
Immunohistochemical staining of human brain tissue using SAPAP3 antibody at 2.5 μg/mL.| Product Name | SAPAP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAPAP3 Antibody: DAP3, SAPAP3, DAP3, Disks large-associated protein 3, DAP-3 |
| Product description | SAPAP3 Antibody: SAP90/PSD-95-associated protein 3 (SAPAP3, also known as DLGAP3) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP3 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). Both SAPAP3 protein and mRNA are targeted to dendrites, whereas SAPAP1, -2, and -4 mRNAs are detected mainly in cell bodies. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. At least two isoforms are known to exist.1) SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem.1997; 272:11943-51. |
| Immunogen | SAPAP3 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human SAPAP3. The immunogen is located within the last 50 amino acids of SAPAP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SAPAP3 antibody can be used for detection of SAPAP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLGAP3 |
|---|---|
| Alternative Names | SAPAP3 Antibody: DAP3 SAPAP3 DAP3 Disks large-associated protein 3 DAP-3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of SAPAP3 in rat brain tissue lysate with SAPAP3 antibody at (A) 1 (B) 2 μg/mL.
Application
Immunofluorescence of SAPAP3 in human brain tissue with SAPAP3 antibody at 20 μg/mL.
Application
Immunohistochemical staining of human brain tissue using SAPAP3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SAPAP3 antibody can be used for detection of SAPAP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAPAP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SAPAP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
