Application
Western blot analysis of lysed extracts from HeLa and MCF cells using SAPK/JNK.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK.| Product Name | SAPK / JNK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JNK2, SAPK, p54a, JNK2A, JNK2B, PRKM9, JNK-55, SAPK1a, JNK2BETA, p54aSAPK, JNK2ALPHA, CDHN, NCAD, JNK2 |
| Product description | Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.1) Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23 Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206D. Margriet Ouwens1, et al. (2002) The EMBO Journal 21: 3782–3793, S Matsuyoshi1, et al. (2006) British Journal of Cancer 94: 532 |
| Immunogen | SAPK/JNK (Ab-183) antibody was raised against a peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK/JNK. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:2000, Immunohistochemistry: 1:50~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK9 |
|---|---|
| Alternative Names | JNK2 SAPK p54a JNK2A JNK2B PRKM9 JNK-55 SAPK1a JNK2BETA p54aSAPK JNK2ALPHA CDHN NCAD JNK2 |
| Molecular Weight(MW) | 46, 54 kDa |
| Tissue Specificity | This antibody detects endogenous level of total SAPK/JNK protein. |
| Entrez Gene | 5601 |
|---|---|
| Protein Accession | NP_001128516.1 |
Application
Western blot analysis of lysed extracts from HeLa and MCF cells using SAPK/JNK.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK.| Application Notes | Western Blot: 1:500~1:2000, Immunohistochemistry: 1:50~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
