Application
Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 μg/mL.Application
Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 μg/mL.| Product Name | SCAMP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCAMP4 Antibody: SCAMP-4, Secretory carrier-associated membrane protein 4, Secretory carrier membrane protein 4 |
| Product description | SCAMP4 Antibody: Secretory carrier membrane proteins (SCAMPs) are integral membrane proteins seen in secretory and endocytic carriers that are thought to play roles in membrane trafficking. One member of this family, SCAMP4, lacks the putative calcium binding and leucine zipper structures as well as the protein-protein interacting NPF domains common to most SCAMP family members. In rats, SCAMP4 mRNA is repressed by progesterone in brain regions associated with female sexual behavior. At least three isoforms of SCAMP4 are known to exist. This SCAMP4 antibody is predicted to not cross-react with any other member of the secretory carrier membrane protein family.1) Hubbard C, Singleton D, Rauch M, et al. The secretory carrier membrane protein family: structure and membrane topology. Mol. Biol. Cell2000; 11:2933-47. |
| Immunogen | SCAMP4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SCAMP4. The immunogen is located within the last 50 amino acids of SCAMP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCAMP4 antibody can be used for detection of SCAMP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCAMP4 |
|---|---|
| Alternative Names | SCAMP4 Antibody: SCAMP-4 Secretory carrier-associated membrane protein 4 Secretory carrier membrane protein 4 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 μg/mL.
Application
Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | SCAMP4 antibody can be used for detection of SCAMP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCAMP4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCAMP4 Antibody is supplied in PBS containing 0.02% sodium azide. |
