Application
Immunocytochemistry of SCARA5 in HepG2 cells with SCARA5 antibody at 2.5 μg/mL.Application
Immunofluorescence of SCARA5 in HepG2 cells with SCARA5 antibody at 20 μg/mL.Application
Western blot analysis of SCARA5 in human liver tissue lysate with SCARA5 antibody at 1 μg/mL.| Product Name | SCARA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCARA5 Antibody: Tesr, NET33, Scavenger receptor class A member 5, Scavenger receptor hlg |
| Product description | SCARA5 Antibody: Scavenger receptor class A member 5 (SCARA5), also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains the collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery.1) Greaves DR, Gough PJ, and Gordon S. Recent progress in defining the role of scavenger receptors in lipid transport, atherosclerosis and host defence. Curr. Op. Lipid. 1998; 9:425-32. |
| Immunogen | SCARA5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SCARA5. The immunogen is located within amino acids 350 - 400 of SCARA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SCARA5 antibody can be used for detection of SCARA5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCARA5 |
|---|---|
| Alternative Names | SCARA5 Antibody: Tesr NET33 Scavenger receptor class A member 5 Scavenger receptor hlg |
| Molecular Weight(MW) | Predicted: 24, 39, 44, 54 kDa Observed: 50 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Tissue Specificity | At least four isoforms of SCARA5 are known to exist; this antibody will detect all four isoforms. |
Application
Immunocytochemistry of SCARA5 in HepG2 cells with SCARA5 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SCARA5 in HepG2 cells with SCARA5 antibody at 20 μg/mL.
Application
Western blot analysis of SCARA5 in human liver tissue lysate with SCARA5 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | SCARA5 antibody can be used for detection of SCARA5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCARA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCARA5 Antibody is supplied in PBS containing 0.02% sodium azide. |
