Application
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.| Product Name | SCF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, blz, Kitlg, contrasted, Kitl, Slf, Kit ligand, Hematopoietic growth factor KL, MGF |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF. Sandwich: To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Kitl |
|---|---|
| Alternative Names | Gb SF Sl Clo Con Mgf SCF SLF blz Kitlg contrasted Kitl Slf Kit ligand Hematopoietic growth factor KL MGF |
Application
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF. Sandwich: To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SCF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
