Application
Immunohistochemistry of SCF in mouse brain tissue with SCF antibody at 2.5 μg/mL.Application
Immunofluorescence of SCF in Human Brain tissue with SCF antibody at 20 μg/mL.Application
Western blot analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SCF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCF Antibody: SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor |
| Product description | SCF Antibody: Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.1) Reber L, Da Silva CA, and Frossard N. Stem cell factor and its receptor c-Kit as targets for inflammatory diseases. Euro. J. Pharmacology 2006; 533:327-40. |
| Immunogen | SCF antibody was raised against an 18 amino acid synthetic peptide from near the center of human SCF. The immunogen is located within amino acids 100 - 150 of SCF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCF antibody can be used for detection of SCF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KITLG |
|---|---|
| Alternative Names | SCF Antibody: SF MGF SCF FPH2 KL-1 Kitl SHEP7 Kit ligand Mast cell growth factor |
| Molecular Weight(MW) | Predicted: 30 kDa Observed: 45 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of SCF in mouse brain tissue with SCF antibody at 2.5 μg/mL.
Application
Immunofluorescence of SCF in Human Brain tissue with SCF antibody at 20 μg/mL.
Application
Western blot analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SCF antibody can be used for detection of SCF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCF Antibody is supplied in PBS containing 0.02% sodium azide. |
