Application
This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.| Product Name | SCF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIT ligand, MGF, Stem cell factor precursor, Mast cell growth factor, SCF, Kit ligand, SF, FPH2, KL-1, Kitl, SHEP7 |
| Immunogen | The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hSCF as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human SCF when used with our biotinylated antigen affinity purified anti-human SCF (XP-5264Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2.0-4.0 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KITLG |
|---|---|
| Alternative Names | KIT ligand MGF Stem cell factor precursor Mast cell growth factor SCF Kit ligand SF FPH2 KL-1 Kitl SHEP7 |
Application
This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human SCF when used with our biotinylated antigen affinity purified anti-human SCF (XP-5264Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2.0-4.0 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SCF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
