Application
Immunofluorescence of SCRN1 in Human Kidney cells with SCRN1 antibody at 20 μg/mL.Application
Immunohistochemistry of SCRN1 in human kidney tissue with SCRN1 antibody at 5 μg/mL.Application
Western blot analysis of SCRN1 in human kidney tissue lysate with SCRN1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SCRN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCRN1 Antibody: SES1, KIAA0193, Secernin-1 |
| Product description | SCRN1 Antibody: SCRN1 was first identified as a cytosolic protein that is involved in the regulation of exocytosis from peritoneal mast cells. More recent studies have shown that SCRN1 expression is upregulated in gastric cancer cell lines and may possess epitopes that could function as tumor-associated antigens, potentially providing targets for cancer vaccines in the treatment of gastric cancers. Another report indicates that decreased expression of SCRN1 via RNAi expression resulted in significantly lower rates of cell growth in colorectal cancer cell lines, and increased SCRN1 expression in patients with colorectal cancer correlated with poor prognosis, suggesting that SCRN1 may also be involved in the regulation of cell growth and might be useful as a prognostic tool.1) Way G, Morrice N, Smythe C, et al. Purification and identification of Secernin, a novel cytosolic protein that regulates exocytosis in mast cells. Mol. Biol. Cell2002; 13:3344-54. |
| Immunogen | SCRN1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human SCRN1. The immunogen is located within amino acids 320 - 370 of SCRN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCRN1 antibody can be used for detection of SCRN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCRN1 |
|---|---|
| Alternative Names | SCRN1 Antibody: SES1 KIAA0193 Secernin-1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Entrez Gene | 9805 |
|---|---|
| Protein Accession | NP_001138985 |
Application
Immunofluorescence of SCRN1 in Human Kidney cells with SCRN1 antibody at 20 μg/mL.
Application
Immunohistochemistry of SCRN1 in human kidney tissue with SCRN1 antibody at 5 μg/mL.
Application
Western blot analysis of SCRN1 in human kidney tissue lysate with SCRN1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | SCRN1 antibody can be used for detection of SCRN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCRN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCRN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
