Application
Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.Application
Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.Application
Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SCUBE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCUBE1 Antibody: Signal peptide, CUB and EGF-like domain-containing protein 1 |
| Product description | SCUBE1 Antibody: SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.1) Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81. |
| Immunogen | SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1. The immunogen is located within amino acids 460 - 510 of SCUBE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCUBE1 |
|---|---|
| Alternative Names | SCUBE1 Antibody: Signal peptide CUB and EGF-like domain-containing protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (93%) |
Application
Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Application
Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Application
Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCUBE1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCUBE1 Antibody is supplied in PBS containing 0.02% sodium azide. |
