Application
Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.Application
Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.Application
Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SCUBE2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCUBE2 Antibody: CEGB1, CEGF1, CEGP1, Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1 |
| Product description | SCUBE2 Antibody: SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.1) Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81. |
| Immunogen | SCUBE2 antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2. The immunogen is located within amino acids 480 - 530 of SCUBE2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SCUBE2 antibody can be used for detection of SCUBE2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCUBE2 |
|---|---|
| Alternative Names | SCUBE2 Antibody: CEGB1 CEGF1 CEGP1 Signal peptide CUB and EGF-like domain-containing protein 2 Protein CEGP1 |
Application
Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
Application
Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
Application
Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SCUBE2 antibody can be used for detection of SCUBE2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCUBE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCUBE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
