Application
Western blot analysis of SCUBE3 in mouse kidney tissue lysate with SCUBE3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of SCUBE3 in human kidney tissue with SCUBE3 antibody at 20 μg/mL.| Product Name | SCUBE3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCUBE3 Antibody: CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3 |
| Product description | SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth.. SCUBE3 has been shown to be an endogenous TGF-β receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-β receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-β1, VEGF, Snail, and Slug.1) Wu BT, Su YH, Tsai MT, et al. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J. Biol. Chem. 2004; 279:37485-90. |
| Immunogen | SCUBE3 antibody was raised against a 15 amino acid synthetic peptide near the center of human SCUBE3. The immunogen is located within amino acids 210 - 260 of SCUBE3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SCUBE3 antibody can be used for detection of SCUBE3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCUBE3 |
|---|---|
| Alternative Names | SCUBE3 Antibody: CEGF3 CEGF3 Signal peptide CUB and EGF-like domain-containing protein 3 |
| Molecular Weight(MW) | 109 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members |
Application
Western blot analysis of SCUBE3 in mouse kidney tissue lysate with SCUBE3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of SCUBE3 in human kidney tissue with SCUBE3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | SCUBE3 antibody can be used for detection of SCUBE3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCUBE3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide. |
