Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colorectal adenocarcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human SDF-1-alpha (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1-alpha.| Product Name | SDF-1a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α (100 ng/mL), a concentration of 2-4 μg/mL of this antibody is required. ELISA: To detect hSDF-1-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha. Sandwich: To detect hSDF-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α. Western Blot: To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL12 |
|---|---|
| Alternative Names | IRH PBSF SDF1 TLSF TPAR1 SCYB12 SDF1A SDF1B Stromal cell-derived factor 1 C-X-C motif chemokine 12 SDF-1 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colorectal adenocarcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human SDF-1-alpha (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1-alpha.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α (100 ng/mL), a concentration of 2-4 μg/mL of this antibody is required. ELISA: To detect hSDF-1-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha. Sandwich: To detect hSDF-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α. Western Blot: To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SDF-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
