Application
Immunocytochemistry of SDHAF1 in 3T3 cells with SDHAF1 antibody at 5 μg/mL.Application
Western blot analysis of SDHAF1 in 3T3 cell lysate with SDHAF1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of SDHAF1 in 3T3 cells with SDHAF1 antibody at 20 μg/mL.| Product Name | SDHAF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SDHAF1 Antibody: SDHAF1 (Succinate dehydrogenase complex assembly factor 1) is one of the subunits of the succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain. SDHAF1 resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Unlike the related protein SDHAF2, SDHAF1 is not thought to be a tumor suppressor.1) Ghezzi D, Goffrini P, Uziel G, et al. SDHAF1, encoding a LYR complex-II specific assembly factor, is mutated in SDH-defective infantile leukoencephalopathy. Nat. Genet. 2009; 41:654-6. |
| Immunogen | SDHAF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SDHAF1. The immunogen is located within the last 50 amino acids of SDHAF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SDHAF1 |
|---|---|
| Alternative Names | SDHAF1 Antibody: LYRM8 LYRM8 Succinate dehydrogenase assembly factor 1 mitochondrial LYR motif-containing protein 8 SDH assembly factor 1 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family members. |
| Entrez Gene | 644096 |
|---|---|
| Protein Accession | NP_001036096 |
Application
Immunocytochemistry of SDHAF1 in 3T3 cells with SDHAF1 antibody at 5 μg/mL.
Application
Western blot analysis of SDHAF1 in 3T3 cell lysate with SDHAF1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of SDHAF1 in 3T3 cells with SDHAF1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SDHAF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SDHAF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
