Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | SDHB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This SDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-234 amino acids from the C-terminal region of human SDHB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SDHB |
|---|---|
| Alternative Names | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrial Iron-sulfur subunit of complex II Ip SDHB SDH SDH1 |
| Molecular Weight(MW) | 32 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
