Application
Immunocytochemistry of SDHD in EL4 cells with SDHD antibody at 2.5 μg/mL.Application
Immunofluorescence of SDHD in EL4 cells with SDHD antibody at 20 μg/mL.Application
Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SDHD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.1) Baysal BE, Ferrell RE, Willett-Brozick JE, et al. Mutations in SDHD, a mitochondrial complex II gene, in hereditary paraganglioma. Science 2000; 287:848-51. |
| Immunogen | SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SDHD |
|---|---|
| Alternative Names | SDHD Antibody: TRAP1 TRAP-1 SDH4 CII-4 CybS |
| Sequence Similarities | 2) Cat. No. 17-207 - EL4 Cell Slide |
| Tissue Specificity | At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms. |
Application
Immunocytochemistry of SDHD in EL4 cells with SDHD antibody at 2.5 μg/mL.
Application
Immunofluorescence of SDHD in EL4 cells with SDHD antibody at 20 μg/mL.
Application
Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SDHD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SDHD Antibody is supplied in PBS containing 0.02% sodium azide. |
