Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | SEMA4C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.1) Wu, H., et al. Eur. J. Cell Biol. 86(6):331-344(2007) |
| Immunogen | This SEMA4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 792-821 amino acids from the C-terminal region of human SEMA4C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEMA4C |
|---|---|
| Alternative Names | Semaphorin-4C SEMA4C KIAA1739 SEMAI |
| Molecular Weight(MW) | 93 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
