Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | SEPSECS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA(sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA(sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA(sec). SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor (Palioura et al., 2009 [PubMed 19608919]).1) Agamy, O., et al. Am. J. Hum. Genet. 87(4):538-544(2010) |
| Immunogen | This SEPSECS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human SEPSECS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEPSECS |
|---|---|
| Alternative Names | O-phosphoseryl-tRNA(Sec) selenium transferase Liver-pancreas antigen LP SLA-p35 SLA/LP autoantigen Selenocysteine synthase Sec synthase Selenocysteinyl-tRNA(Sec) synthase Sep-tRNA:Sec-tRNA synthase SepSecS Soluble liver antigen SLA UGA suppress |
| Molecular Weight(MW) | 56 kDa |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
