Application
Immunocytochemistry of SEPT1 in Raji cells with SEPT1 antibody at 5 μg/mL.Application
Immunofluorescence of SEPT1 in Raji cells with SEPT1 antibody at 20 μg/mL.Application
Western blot analysis of SEPT1 in Raji cell lysate with SEPT1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | SEPT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEPT1 Antibody: LARP, SEP1, DIFF6, PNUTL3, Septin-1, LARP |
| Product description | SEPT1 Antibody: SEPT1 (also known as Septin 1), is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. SEPT1 was initially identified through yeast two-hybrid screening as an interaction partner for the serine/threonine kinase Aurora-B. During mitosis, SEPT1 localizes to the spindle pole, suggesting that it may play a role in cytokinesis and chromosome congression.1) Qi M, Yu W, Liu S, et al. Septin1, a new interaction partner for human serine/threonine kinase aurora-B. Biochem. Biophys. Res. Commun. 2005; 336:994-1000. |
| Immunogen | SEPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SEPT1. The immunogen is located within amino acids 300 - 350 of SEPT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SEPT1 antibody can be used for detection of SEPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | 1-Sep |
|---|---|
| Alternative Names | SEPT1 Antibody: LARP SEP1 DIFF6 PNUTL3 Septin-1 LARP |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
| Tissue Specificity | At least two isoforms of SEPT1 are known to exist. This antibody is predicted to not cross-react with other Septin protein family members. |
Application
Immunocytochemistry of SEPT1 in Raji cells with SEPT1 antibody at 5 μg/mL.
Application
Immunofluorescence of SEPT1 in Raji cells with SEPT1 antibody at 20 μg/mL.
Application
Western blot analysis of SEPT1 in Raji cell lysate with SEPT1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | SEPT1 antibody can be used for detection of SEPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SEPT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SEPT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
