Application
Western blot analysis of SEPT2 using rabbit polyclonal SEPT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SEPT2 gene.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | SEPT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Septin-2, Neural precursor cell expressed developmentally down-regulated protein 5, NEDD-5, SEPT2, DIFF6, KIAA0158, NEDD5 |
| Product description | Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.1) Martins-de-Souza, D., et al.Psychiatr Res (2010) In press : |
| Immunogen | This SEPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-309 amino acids from the C-terminal region of human SEPT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEPT2 |
|---|---|
| Alternative Names | Septin-2 Neural precursor cell expressed developmentally down-regulated protein 5 NEDD-5 SEPT2 DIFF6 KIAA0158 NEDD5 |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of SEPT2 using rabbit polyclonal SEPT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SEPT2 gene.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM290870