SEPT2 is required for normal progress through mitosis. SEPT2 is involved in cytokinesis.1) Spiliotis, E.T., (2008) J. Cell Biol. 180 (2), 295-303.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Septin 2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
Septin 2 antibody can be used for detection of Septin 2 by ELISA at 1:312500. Septin 2 antibody can be used for detection of Septin 2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
Septin 2 antibody can be used for detection of Septin 2 by ELISA at 1:312500. Septin 2 antibody can be used for detection of Septin 2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store Septin 2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.