Application
SERPINH1 Antibody (RB18952) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of SERPINH1 antibody in HepG2 cell line lysates (35ug/lane)| Product Name | SERPINH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Colligin, Rheumatoid arthritis-related antigen RA-A47, SERPINH1, CBP1, CBP2, HSP47, SERPINH2 |
| Product description | SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.1) Araki,K., Br. J. Cancer 101 (3), 492-497 (2009) |
| Immunogen | This SERPINH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-196 amino acids from the Central region of human SERPINH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SERPINH1 |
|---|---|
| Alternative Names | Serpin H1 47 kDa heat shock protein Arsenic-transactivated protein 3 AsTP3 Cell proliferation-inducing gene 14 protein Collagen-binding protein Colligin Rheumatoid arthritis-related antigen RA-A47 SERPINH1 CBP1 CBP2 HSP47 SERPINH2 |
| Molecular Weight(MW) | 46 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
SERPINH1 Antibody (RB18952) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of SERPINH1 antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
