WB
Western blot analysis of HSP47/SERPINH1 using anti-HSP47/SERPINH1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP47/SERPINH1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HSP47/SERPINH1 using anti-HSP47/SERPINH1 antibody. HSP47/SERPINH1 was detected in a paraffin-embedded section of human esophageal squamous cancer tissue. The tissue section was incubated with rabbit anti-HSP47/SERPINH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of HSP47/SERPINH1 using anti-HSP47/SERPINH1 antibody. HSP47/SERPINH1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-HSP47/SERPINH1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A431 cells using anti-HSP47/SERPINH1 antibody. Overlay histogram showing A431 cells stained with Rabbit polyclonal antibody to HSP47/SERPINH1 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSP47/SERPINH1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to HSP47/SERPINH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SERPINH1 |
|---|---|
| Gene Synonyms | CBP1 CBP2 OI10 gp46 AsTP3 HSP47 PIG14 PPROM RA-A47 SERPINH2 |
| Gene Full Name | serpin family H member 1 |
| Gene Summary | This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
| Alternative Names | 47 kDa heat shock protein AsTP3 CBP1 CBP2 Colligin gp46 HSP47 PIG14 PPROM RA A47 Serpin H1 SERPINH1 SERPINH2 |
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Endoplasmic reticulum. |
WB
Western blot analysis of HSP47/SERPINH1 using anti-HSP47/SERPINH1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP47/SERPINH1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HSP47/SERPINH1 using anti-HSP47/SERPINH1 antibody. HSP47/SERPINH1 was detected in a paraffin-embedded section of human esophageal squamous cancer tissue. The tissue section was incubated with rabbit anti-HSP47/SERPINH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of HSP47/SERPINH1 using anti-HSP47/SERPINH1 antibody. HSP47/SERPINH1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-HSP47/SERPINH1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A431 cells using anti-HSP47/SERPINH1 antibody. Overlay histogram showing A431 cells stained with Rabbit polyclonal antibody to HSP47/SERPINH1 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSP47/SERPINH1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
