Application
IHC-P analysis of human colon tissue using HSP47 antibody [EPR4217] at a dilution of 1:300. Antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Tissue counterstained with Hematoxylin.Application
WB analysis of (1) A431 and (2) NIH/3T3 lysates (20 μg per lane) using HSP47 antibody [EPR4217] at a dilution of 1:2000.| Product Name | HSP47 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR4217] to HSP47HSP47 is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. This protein localizes to the endoplasmic reticulum lumen and binds collagen; thus, it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to HSP47 have been found in patients with rheumatoid arthritis. |
| Immunogen | A synthetic peptide corresponding to residues in human HSP47 was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR4217 |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| IHC-P: 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB: 1/1000 - 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | RA-A47 SERPINH1 AsTP3 gp46 CBP1 RAA47 PPROM HSP47 SERPINH2 CBP2 RA A47 |
|---|---|
| Target | HSP47 |
| Molecular Weight(MW) | 46 kDa (note) |
Application
IHC-P analysis of human colon tissue using HSP47 antibody [EPR4217] at a dilution of 1:300. Antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Tissue counterstained with Hematoxylin.
Application
WB analysis of (1) A431 and (2) NIH/3T3 lysates (20 μg per lane) using HSP47 antibody [EPR4217] at a dilution of 1:2000.| Application Notes | IHC-P: 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB: 1/1000 - 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
