Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in NCI-H460,HL-60 cell line lysates (35ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).Application
Western blot analysis of Sestrin-1 antibody in mouse heart tissue lysates (35ug/lane)| Product Name | Sestrin-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sestrin-1 is involved in the reduction of peroxiredoxins. This protein may also be regulator of cellular growth.1) Budanov,A.V., Science 304 (5670), 596-600 (2004) |
| Immunogen | This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-341 amino acids from the C-terminal region of human Sestrin-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SESN1 |
|---|---|
| Alternative Names | Sestrin-1 p53-regulated protein PA26 SESN1 PA26 SEST1 |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in NCI-H460,HL-60 cell line lysates (35ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).
Application
Western blot analysis of Sestrin-1 antibody in mouse heart tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
