Application
Immunohistochemistry of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 5 μg/ml.Application
Immunofluorescence of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 20 μg/ml.Application
Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | SESTRIN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).1) Budanov AV, Shoshani T, Faerman A, et al. Identification of a novel stress-responsive gene Hi95 involved in regulation of cell viability. Oncogene 2002; 21:6017-31. |
| Immunogen | SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2. The immunogen is located within amino acids 220 - 270 of SESTRIN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SESN2 |
|---|---|
| Alternative Names | SESTRIN-2 SES2 SEST2 SESN2 HI95 |
| Molecular Weight(MW) | Predicted: 54 kDa Observed: 53 kDa |
| Sequence Similarities | 2) Cat. No. 1305 - Human Kidney Tissue Lysate |
| Tissue Specificity | SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist; this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1. |
Application
Immunohistochemistry of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 5 μg/ml.
Application
Immunofluorescence of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 20 μg/ml.
Application
Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SESTRIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SESTRIN2 antibody is supplied in PBS containing 0.02% sodium azide. |
