Application
Western blot analysis of SETDB1 using rabbit polyclonal SETDB1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2).Application
Western blot analysis of anti-SETDB1 Pabin Ramos cell line lysate (35ug/lane).| Product Name | SETDB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, ESET, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SETDB1, KIAA0067, KMT1E |
| Product description | The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.1) Ichimura, T., et al., J. Biol. Chem. 280(14):13928-13935 (2005). |
| Immunogen | This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1193-1225 amino acids from the C-terminal region of human SETDB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SETDB1 |
|---|---|
| Alternative Names | Histone-lysine N-methyltransferase SETDB1 ERG-associated protein with SET domain ESET Histone H3-K9 methyltransferase 4 H3-K9-HMTase 4 Lysine N-methyltransferase 1E SET domain bifurcated 1 SETDB1 KIAA0067 KMT1E |
| Molecular Weight(MW) | 143 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of SETDB1 using rabbit polyclonal SETDB1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2).
Application
Western blot analysis of anti-SETDB1 Pabin Ramos cell line lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
