Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: mouse spleen lysate Lane 6: mouse testis lysate Lane 7: rat lung lysate Lysates/proteins at 20 ug per lane.| Product Name | SF3B2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq].1) Matsuoka, S., et al. Science 316(5828):1160-1166(2007) |
| Immunogen | This SF3B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-568 amino acids from the Central region of human SF3B2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SF3B2 |
|---|---|
| Alternative Names | Splicing factor 3B subunit 2 Pre-mRNA-splicing factor SF3b 145 kDa subunit SF3b145 SF3b150 Spliceosome-associated protein 145 SAP 145 SF3B2 SAP145 |
| Molecular Weight(MW) | 100 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: mouse spleen lysate Lane 6: mouse testis lysate Lane 7: rat lung lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
